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Immunofluorescence staining to observe the colocalization of RDYH58-exo and unm-exo with <t>epithelial</t> cells, and endothelial cells. (A) Colocalization images of <t>anti-EpCAM</t> antibody for epithelial cells and exosomes. (B, D) The Pearson’ R value indicates the degree of colocalization. (C) Colocalization images of anti-CD31 antibody for endothelium and exosomes. (E) The Pearson’ R value indicates the degree of colocalization between RDYH58-exo and myofibroblasts, macrophages, epithelial cells, and endothelial cells ( n = 3‒5) (∗ ∗∗P < 0.001).
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Immunofluorescence staining to observe the colocalization of RDYH58-exo and unm-exo with <t>epithelial</t> cells, and endothelial cells. (A) Colocalization images of <t>anti-EpCAM</t> antibody for epithelial cells and exosomes. (B, D) The Pearson’ R value indicates the degree of colocalization. (C) Colocalization images of anti-CD31 antibody for endothelium and exosomes. (E) The Pearson’ R value indicates the degree of colocalization between RDYH58-exo and myofibroblasts, macrophages, epithelial cells, and endothelial cells ( n = 3‒5) (∗ ∗∗P < 0.001).
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Immunofluorescence staining to observe the colocalization of RDYH58-exo and unm-exo with <t>epithelial</t> cells, and endothelial cells. (A) Colocalization images of <t>anti-EpCAM</t> antibody for epithelial cells and exosomes. (B, D) The Pearson’ R value indicates the degree of colocalization. (C) Colocalization images of anti-CD31 antibody for endothelium and exosomes. (E) The Pearson’ R value indicates the degree of colocalization between RDYH58-exo and myofibroblasts, macrophages, epithelial cells, and endothelial cells ( n = 3‒5) (∗ ∗∗P < 0.001).
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Immunofluorescence staining to observe the colocalization of RDYH58-exo and unm-exo with epithelial cells, and endothelial cells. (A) Colocalization images of anti-EpCAM antibody for epithelial cells and exosomes. (B, D) The Pearson’ R value indicates the degree of colocalization. (C) Colocalization images of anti-CD31 antibody for endothelium and exosomes. (E) The Pearson’ R value indicates the degree of colocalization between RDYH58-exo and myofibroblasts, macrophages, epithelial cells, and endothelial cells ( n = 3‒5) (∗ ∗∗P < 0.001).

Journal: Acta Pharmaceutica Sinica. B

Article Title: RDYH58 functional exosomes targeting myofibroblasts loaded with siFKBP10 for inhibition of collagen biosynthesis and secretion of IPF

doi: 10.1016/j.apsb.2025.08.017

Figure Lengend Snippet: Immunofluorescence staining to observe the colocalization of RDYH58-exo and unm-exo with epithelial cells, and endothelial cells. (A) Colocalization images of anti-EpCAM antibody for epithelial cells and exosomes. (B, D) The Pearson’ R value indicates the degree of colocalization. (C) Colocalization images of anti-CD31 antibody for endothelium and exosomes. (E) The Pearson’ R value indicates the degree of colocalization between RDYH58-exo and myofibroblasts, macrophages, epithelial cells, and endothelial cells ( n = 3‒5) (∗ ∗∗P < 0.001).

Article Snippet: The sections were blocked for 60 min and incubated overnight at 4 °C with antibodies against Epithelial Cell Adhesion Molecule (Proteintech, CL555-21050), CD68 (Proteintech, CL594-25747), Platelet Endothelial Cell Adhesion Molecule-1 (Proteintech, CL647-65058), and α -SMA (Cell Signaling, P62736 ).

Techniques: Immunofluorescence, Staining