Journal: Nature Communications

Article Title: Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State

doi: 10.1038/s41467-026-68909-z

Figure Lengend Snippet: a Schematic outline of p300 CUT&Tag analysis in BLM-treated FD-AOs and representative transcription factor motifs enriched in p300 peaks significantly increased (adjusted p value < 0.05) upon BLM treatment. Created in BioRender. Tsutsui, Y. (2026) https://BioRender.com/tzol3zv . Significantly increased peaks were identified using DiffBind. Motif enrichment was assessed using HOMER. b Representative p300 and H3K27ac CUT&Tag tracks visualized in IGV at injury-responsive epithelial genes ( CDKN1A , MMP7 , CLDN4 , and S100A2 ) in FD-AOs treated with DMSO or BLM. Signal intensities were normalized to E. coli DNA . Colored bars indicate genomic regions containing transcription factor motifs of interest. c Whole-well imaging of FD-AOs treated with BLM from days 11 to 14, followed by treatment with AP-1 inhibitors (SR11302, 10 μM; T-5224, 40 μM) from days 14 to 17. Scale bars: 2 mm. d Quantification of the matrix areas. Data are presented as mean ± SEM. One-way ANOVA followed by Tukey’s multiple comparisons test: ** p < 0.01, **** p < 0.0001 ( n = 3 biologically independent experiments). e Schematic outline of siRNA-based gene silencing in the micro-patterned culture. Created in BioRender. Tsutsui, Y. (2026) https://BioRender.com/35r9mk4 f Gene expression data of ATF3 and HNF1B in the micro-patterned culture. Data are presented as mean ± SEM. n = 4 (siCont), n = 3 (siATF3), and n = 4 (siHNF1B) biologically independent experiments. One-way ANOVA followed by Tukey’s multiple comparisons test; *** p < 0.001, **** p < 0.0001. g Gene expression data of ATCS markers. Data are presented as mean ± SEM. n = 4 (siCont), n = 3 (siATF3), and n = 4 (siHNF1B) biologically independent experiments. One-way ANOVA followed by Tukey’s test; ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The dissociated cells were stained with mouse anti-human EpCAM antibody (Santa Cruz Biotechnology, SC-66020) at a 1:100 dilution to isolate epithelial cells and fibroblasts.

Techniques: Imaging, Gene Expression

Journal: Nature Communications

Article Title: Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State

doi: 10.1038/s41467-026-68909-z

Figure Lengend Snippet: Working model of p300-mediated regulation of iATCs differentiation and epithelial–fibroblast crosstalk.

Article Snippet: The dissociated cells were stained with mouse anti-human EpCAM antibody (Santa Cruz Biotechnology, SC-66020) at a 1:100 dilution to isolate epithelial cells and fibroblasts.

Techniques: